Mass Cytometry
Driving groundbreaking insights at the single-cell level, millions of times
Mass cytometry, powered by CyTOF® technology, is the world’s most advanced cytometric platform. It has opened new doors to discovery and clinical research by empowering investigators to interrogate more than 50 markers simultaneously on millions of individual cells. Join the hundreds who have reimagined cytometry with CyTOF to unveil new cell types, functions and biomarkers in immunology, cancer, infectious disease and more.
Workflow
1

Design panel
Easily build panels with up to 50 metal-tagged antibodies and reagents using Maxpar® Panel Designer. Add barcoding to multiplex your samples for even better data quality. Or use pre-built panels to get started!
2

Prepare samples
CyTOF systems provide highly multiplexed single-cell data from cell suspension samples. Standard protocols for preparing single-cell suspensions from solid or liquid tissues and tumors can be used for CyTOF sample prep. Check out our support page for staining protocols.
3

Acquire data
The CyTOF XT™ system has fully automated pellet resuspension, calibration bead addition and acquisition of sample batches from the Autosampler carousel.
The Helios™ system provides the same excellent standard of data quality as CyTOF XT but requires manual resuspension and loading of samples.
4

Analyze data
CyTOF systems produce standard FCS 3.0 (Helios) or 3.1 (CyTOF XT) data files that can be analyzed with any compatible software, including FCS Express™ and Maxpar Pathsetter™.
PUBLICATIONS TRENDING NOW
Explore these compilations of recent mass cytometry publications focused on specific areas of investigation.

January 2022: COVID-19 research update
November 2021: CAR T cells
July 2021: The value of high-parameter cytometry
April 2021: Immuno-oncology
January 2021: Cancer vaccine development
November 2020: Vaccines for infectious disease
July 2020: COVID-19 disease research
Browse the latest research highlighting mass cytometry technology.
VIEW ALLAPPLICATIONS
Apr 08 - Apr 13
Location
AACR Annual Meeting 2022 (American Association for Cancer Research) in New Orleans, LA
Mar 27 - Mar 30
Location
ABRF (Association of Biomolecular Resource Facilities) in Palm Springs, CA
Mar 23
Webinar
Sample Tracking in Genomic Workflows Using the Biomark ™ X System and New Analysis Pipeline Software
Jun 21 - Jun 23
Location
Sequencing to Function: Analysis and Applications for the Future (SFA²F) in Santa Fe, NM
Oct 12 - Oct 14
Location
NERLSCD 2022 (Northeast Regional Laboratory Staff & Core Directors) in Rochester, NY
Sep 16 - Sep 18
Location
GLIIFCA XXXI (Great Lakes International Imaging and Flow Cytometry Association) in Indianapolis, IN
Apr 27
Virtual
Scientist in the Spotlight - Receptor Occupancy - Gerd Bringeland and Sonia Gavasso "Monitoring antibody therapies – bead based receptor occupancy quantitation in mass cytometry"
Apr 25
Webinar
CAP Today: Innovations in Immune Profiling for Clinical Research
Apr 14
Virtual
Scientist in the Spotlight - Simon Cabello and Julie Vendrell-"Theranostic, prognostic and diagnostic uses of a flexible Solid Tumor NGS library prep assay in a university hospital
May 18
Virtual
Scientist in the Spotlight in collaboration with the EACR - Bernd Bodenmiller "Multiscale Analysis of Tumor Ecosystems Towards Precision Medicine"
Jun 07
Virtual
Scientist in the Spotlight - Ilaria Michelozzi High-dimensional functional phenotyping of preclinical human CAR T cells using mass cytometry
May 24
Webinar
Save the Date: Igniting Insight in Immuno-Oncology CyTOF® Flow Cytometry Virtual Summit
Jun 14
Webinar
Save the Date! Cytometry Powered by Time-of-Flight: Igniting Insight into Immuno-Oncology: CyTOF Summit, Tuesday, June 14
Jun 15
Webinar
Save the Date! Cytometry Powered by Time-of-Flight: Igniting Insight into Immuno-Oncology: IMC Forum, Wednesday, June 15
Dec 07
Virtual
Emerging Approaches to Improving Adoptive Cell Therapy
Greg Hopkins
Matthew Spitzer, PhD
Emily Thrash, PhD
Jonathan Caron, PhD
Lior Goldberg, MD
Feb 14
Virtual
Singapore Immunology Network & Fluidigm Greater Asia High-Dimensional (HiDi) Immune Profiling Research Award 2021 - Award Winners
Mar 25
Virtual
GEN: Single-Cell Immune Profiling Can Help Resolve Tumor Microenvironment Complexities
Apr 08
Virtual
Scientist in the Spotlight - Nicole Strittmater "Multimodal imaging strategies to increase our understanding of drug delivery"
Nov 13 - Nov 14
Physical-and-Virtual
SITC 2021 (Society for Immunotherapy of Cancer) in Washington, DC
Nov 01 - Nov 03
Virtual
Frontiers in Cancer Science 2021
Gerard Evan
Jesús Gil Valles
Thomas Gajewski
Nov 07 - Nov 10
Virtual
Australasian Cytometry Society Conference
Nov 17
Virtual
Mass Cytometry versus Spectral Flow Cytometry: A User’s Perspective
Paula Niewood
Marjolijn Hameetman
Mar 03
Virtual
Biocompare : How to Best Utilize Innovations in Multi-Omics Flow Cytometry
Resources
Webinars & videos
Bibliographies
Flyers & brochures
Spotlight articles
Technical documents
User guides
Protocols
Tools
Unless explicitly and expressly stated otherwise, all Fluidigm products are provided for Research Use Only, not for use in diagnostic procedures. For more information see https://www.fluidigm.com/legal/notices.